Confused by qPCR?

Master qPCR

From PCR Fundamentals to Reliable Results

Absolute Quantification

Investment: 396 €

Online Course | Instant Access | Self-paced |Certificate | Makes real-time PCR clear, practical, and doable

Want to truly understand qPCR - not just follow protocols?

Go beyond protocols and develop a deep, practical understanding of qPCR - one of the most essential technologies in molecular biology, diagnostics, and biotechnology.

This comprehensive training is designed to help you confidently design, set up, analyze, and troubleshoot qPCR experiments with clarity and precision. From PCR fundamentals to quantification strategies, data interpretation, and MIQE 2.0 best practices, you’ll gain the knowledge used in real-world laboratories worldwide.

Learn through practical insights, workflow-focused guidance, and expert instruction from a globally experienced qPCR trainer.

What you’ll learn:

What PCR is and how it works
Key differences between PCR and qPCR
How to interpret amplification plots, baseline, threshold, and Cq values
Fluorescence-based detection: dyes vs. probes
Understand absolute quantification in practice
How to build, evaluate, and troubleshoot standard curves
Critical aspects of experimental setup and assay design - step by step
How to choose the right qPCR system for your laboratory needs
Common qPCR mistakes that affect data quality - and how to avoid them
Essentials of MIQE 2.0 guidelines, qPCR data quality, and reporting
Practical best practices for generating more reliable and reproducible qPCR results

Bonus: Instrument selection guide

Extras: Printable checklist + quizzes

Certificate of completion and access for 6 months

Benefit: Upgrade your skills and save - 20% off your next course.

Who this training is for:

Students and researchers building confidence in qPCR
Laboratory technicians and molecular biology professionals
Scientists who want to improve data quality and experimental consistency
Professionals working in academic, biotech, pharma, and molecular testing laboratories
Anyone seeking practical qPCR knowledge beyond theoretical concepts

What You’ll Gain

Clear understanding of the complete qPCR workflow
Confidence in designing robust and reproducible qPCR experiments
Practical laboratory knowledge applicable across academic, diagnostic, biotech, and pharmaceutical settings
Expert insights from extensive global hands-on qPCR training experience
Certificate of completion to showcase your new skills and achievement

Course Curriculum

Module 1 PCR vs qPCR: Understanding the Core Principles

Build a solid foundation in amplification biology and clearly understand what sets real-time qPCR apart from conventional PCR in modern molecular applications.

Module 2 qPCR Detection & Quantification Strategies

Learn how qPCR data is generated and interpreted in practice - including amplification plots, baseline, threshold, Cq values, fluorescence chemistry, absolute quantification, and standard curve analysis.

Module 3 Experimental Setup, Workflow & Best Practices

Learn how to design robust qPCR experiments, structure your workflow efficiently, and avoid the most common sources of variability and error.

Module 4 Choosing the Right qPCR System for Your Lab

Understand how to evaluate and select a qPCR platform that fits your applications, throughput, and laboratory environment.

Module 5 MIQE Guidelines - Modern Standards for qPCR Reporting

Learn how to apply MIQE principles to ensure high-quality, transparent, and publication-ready qPCR data reporting.

Knowledge Checks & Interactive Quizzes

Reinforce your understanding with practical quizzes and knowledge checks designed to help you apply key concepts and build confidence throughout the course.

About me

Kasia Ewert, PhD - course director

I’m a qPCR specialist with global training experience in molecular technologies. I spent over a decade at Thermo Fisher Scientific, supporting laboratories in the effective implementation and optimization of qPCR methods across a wide range of applications.

I also gained research experience in the United Kingdom, including at the University of Cambridge, which allows me to combine strong scientific foundations with practical, real-world laboratory expertise.

''I’ve trained hundreds of qPCR users worldwide and know the common pitfalls - this self-paced course was carefully designed by me to help you master qPCR with confidence and precision.''

Is this course for the beginners?

Do I need a specific qPCR instrument?

Is the course available in other languages?

Why does the course materials available for 6 months?

Ready to Transform Your qPCR Skills?

Learn how to confidently design, run, and analyze qPCR experiments with reliable and reproducible results.

Enroll in the Complete qPCR Course

Price: 396 €

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